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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. HbsAg is positive in acute and chronic Hepatitis B infections, since the antigen is found on the actual surface of the virus. HbeAg is present in the blood when the hepatitis B viruses are replicating, indicating an active infection. Anti-Hbc IgM is present due to the immune response to the presence of the hepatitis core antigen and indicates an acute infection. Anti-HBs is generally interpreted as indicating recovery and immunity from hepatitis B virus infection, according to the CDC.
Given the following results, what is the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative

A) susceptible
B) chronic infection
C) acute infection
D) immunization

2. Creatinine clearance tests are utilized to estimate the glomerular function of the kidney. The creatitine clearance calculation is defined as the volume of plasma that is cleared of creatinine by the kidney per unit of time and uses the following formula:
creatinine clearance = Urine creatinine conc x Volume / Plamsa creatinine conc Creatinine clearance measurements have a recommended specimen requirement = 24-hour urine collection.
Which of the following tests would be useful in the assessment of glomerular filtration:

A) Urea clearance
B) PSP test
C) Creatinine clearance
D) 24 hour urine protein

3. This antibody is found in the serum of Le(a-b-) secretors.
Which one of these Lewis blood group system phenotypes usually produces anti-Lea?

A) Le(a+b-)
B) Le(a--b-)
C) Le(a--b+)
D) Le(a+b+)

4. Phase of reactivity is primarily at immediate spin (4+) and reactions get weaker at AHG (w+). There is no specific pattern of reactivity and the auto control is negative which rules out an autoantibody. This is a strong cold antibody which is still slightly present after incubation and washing.
Activation and binding of the antibody takes place at room temperature or colder. Eliminating this phase will prevent the antibody from binding. Cold antibodies usually are more of a nuisance to blood bankers and are not clinically significant.
When performing an antibody screen, both the screen cells are 4+ at immediate spin and W+ at AHG. The antibody panel shows 4+ reactions at immediate spin and W+ reactions at AHG and there is no specific match to the reaction pattern. The auto control is negative. What would be a logical next step?

A) Repeat testing using warmed patient sample and reagents and just do AHG reading
B) Have patient redrawn
C) Run an enzyme panel

5. Troponin is potentially more specific for myocardial damage than CK-MB and stays elevated longer. It may eventually replace CK-MB as the standard marker of myocardial damage. In addition, troponin T has been known to be elevated in the setting of even mild degrees of renal failure. Troponin and CK-MB both tend to rise approximately 3 hours after a MI (hence why the correct answer is "A", since it is false); however, troponins can stay elevated up to 2 weeks as CK-MB tends to return to baseline around 36 hours.
Chemistry
Which of the following statements is FALSE regarding troponin?

A) It rises much sooner after an MI than CK-MB
B) It is potentially more specific than CK-MB
C) It stays positive much longer than CK-MB
D) Troponin T is often elevated in renal failure patients

Solutions: